Carpe's Garden

当我们在写一个比较复杂的脚本时，往往会涉及到很多小的函数，但是实现其功能的写法有很多种，如果能够清楚他们的速度，那么又便于提升我们代码整体的效率。

举一个非常简单而又常用的例子，用python写一个转化fasta文件的反向互补序列的脚本。

实现的方法有很多种，我们这里只讨论3中比较常用的：

1. replace()
2. dictionary
3. transtable()

个人整理了一些排球的入门资料，仅供有兴趣的小伙伴学习和分享。

发现一个endnote导入文件的bug,我们首先来看一下一般的导入操作：

用endnote导入google scholar的参考文献，导出格式为Endnote格式

以一篇文章刚发表在bioRxiv上的文章为例：

1

MitoZ: A toolkit for mitochondrial genome assembly, annotation and visualization

基础知识

单细胞测序扫盲：是什么？为什么？怎么做？
单细胞测序综述
单细胞测序教程
单细胞测序方法比较

blogs

Cell：利用人工智能绘制衰老大脑的基因表达图谱
两篇Cell，五篇Science从癌症到发育， 从衰老到免疫，单细胞研究显神威

background

I got a long list of animals, about 9000, and I need to figure out where each animal lives, specially whether lives in Africa. Unfortunately, there is no one database containing this kind information, all website I can got from internet is something like common knowledge other than a scientific list. Finally, I realized I forget a powful tool - wikipedia, but how to make it? I can not search them one by one, it must kill me. wait, I can code to solve this problem, come on, I am a bioinformatican.

ERROR

Parse error for this part of the blast XML document: Error on line 1042356: The entity name must immediately follow the ‘&’ in the entity reference.

When I use diamond replacing blast to search NR database, the result with xml format can’t be parsed by blast2GO. Aftering see this page, I got it soloved.